夏井坂 光輝

夏井坂 光輝

客員研究員

学歴

1993年3月
北嶺中高等学校 卒業
1999年3月
北海道大学医学部医学科 卒業
2007年3月
北海道大学大学院医学研究科
病態制御学専攻博士課程 修了

職経

1999年6月
北海道大学医学部付属病院 内科研修医
2000年4月
市立旭川病院 内科研修医
2001年4月
札幌厚生病院 消化器科 医師
2002年4月
市立稚内病院 内科 医師
2003年4月
北海道大学病院 第三内科 医師
2007年4月
札幌北楡病院 消化器科 医師
2008年10月
Pennsylvania大学 消化器科 博士研究員
2012年6月
北海道大学病院 消化器内科 助教
2014年4月
北海道大学病院 消化器内科 医員
2014年5月
北海道大学大学院医学研究科
消化器内科 特任研究助教
2014年10月
北海道大学病院 消化器内科 助教

所属学会

  • 日本内科学会
  • 日本消化器病学会
  • 日本消化器内視鏡学会
  • 日本肝臓学会
  • 日本臨床腫瘍学会
  • 日本癌学会
  • アメリカ消化器病学会
  • アメリカ癌学会

認定医 / 専門医

  • 日本内科学会 認定内科医
  • 日本消化器病学会 消化器病専門医
  • 日本消化器内視鏡学会 消化器内視鏡専門医
  • 日本肝臓学会 肝臓専門医

研究費等

  • 厚生労働科学研究費補助金(肝炎等克服緊急対策研究事業)「ゲノム網羅的解析によるB型肝炎ウィルス感染の病態関連遺伝子の同定と新規診断法の開発」 研究分担者 (2014年度)
  • 科学研究費助成事業 基盤研究(C)「食道癌三次元培養モデルを用いた癌幹細胞ニッチの解析」 研究分担者 (2014-2016年度)
  • 寿原記念財団研究助成金 (2013年度)
  • 科学研究費助成事業 若手研究(B)「食道扁平上皮癌幹細胞を標的とした新規治療法の開発」 研究代表者 (2013-2014年度)
  • 厚生労働科学研究費補助金(肝炎等克服緊急対策研究事業)「次世代シーケンシング・ゲノムワイド関連解析を用いたC型肝炎治療に伴う肝病態進展軽快、肝発癌に関わる宿主因子の解析」 研究分担者 (2013-2014年度)
  • 厚生労働科学研究費補助金(肝炎等克服緊急対策研究事業)「B型肝炎ウイルス感染の病態別における宿主因子等について、網羅的な遺伝子解析を用い、新規診断法及び治療法の開発を行う研究」 研究分担者 (2013年度)
  • 北海道大学総長室事業推進経費 「若手研究者自立支援」 (2013年度)
  • 日本消化器病学会「若手研究者のための国際学会参加費用一部助成」 (2013年度)

論文(英文のみ)

  1. Kinugasa H, Whelan KA, Tanaka K, Natsuizaka M, Long A, Guo A, Chang S, Kagawa S, Srinivasan S, Guha M, Yamamoto K, St. Clair DK, Avadhani NG, Diehl JA, Nakagawa H.
    Mitochondrial SOD2 regulates epithelial-mesenchymal transition and cell populations defined by differential CD44 expression.
    Oncogene. 2014. In press.
  2. Kagawa S*, Natsuizaka M* (* contributed equally), Whelan KA, Facompre N, Naganuma S, Ohashi S, Kinugasa H, Egloff AM, Basu D, Gimotty PA, Klein-Szanto AJ, Bass A, Wong K, Diehl JA, Rustgi AK, Nakagawa H.
    Cellular senescence checkpoint function determines differential Notch1-dependent oncogenic and tumor suppressor activities.
    Oncogene. 2014. In press.
  3. Suda G, Yamamoto Y, Nagasaka A, Furuya K, Kudou M, Chuganji Y, Tsukuda Y, Tsunematsu S, Sato G, Terashita K, Nakai M, Horimoto H, Sho T, Natsuizaka M
  4. , Ogawa K, Ohnishi S, Chuma M, Fujita Y, Abe R, Taniguchi M, Nakagawa M, Asahina Y, Sakamoto N for the NORTE study group.
    Serum granulysin levels as a predictor of serious telaprevir-induced dermatological reaction.
    Hepatol Res. 2014. In press.
  5. Kubota Y, Kawakami H, Natsuizaka M, Kawakubo K, Marukawa K, Kudo T, Abe Y, Kubo K, Kuwatani M, Hatanaka Y, Mitsuhashi T, Matsuno Y, Sakamoto N.
    CTNNB1 mutational analysis of solid-pseudopapillary neoplasms of the pancreas using EUS-guided fine-needle aspiration and next-generation deep sequencing.
    J Gastroenterol. 2014. In press.
  6. Tsunematsu S, Natsuizaka M, Fujita H, Otsuka N, Terashita K, Sato F, Kobayashi T, Nakai M, Tsukuda Y, Horimoto H, Sho T, Suda G, Nakanishi M, Hashino S, Chuma M, Sakamoto N.
    Hepatosplenic Gamma-delta T-cell Lymphoma Associated with Epstein-Barr Virus.
    Intern Med. 2014. 2014;53(18):2079-82
  7. Natsuizaka M, Kinugasa H, Kagawa S, Whelan KA, Naganuma S, Subramanian H, Chang S, Nakagawa KJ, Rustgi NL, Kita Y, Natsugoe S, Basu D, Gimotty PA, Klein-Szanto AJ, Diehl JA, Rustgi1 AK and Nakagawa H.
    IGFBP3 promotes esophageal cancer growth by suppressing oxidative stress in hypoxic tumor microenvironment.
    Am J Cancer Res. Jan 15;4(1):29-41. 2014.
  8. Chuma M, Sakamoto N, Nakai A, Hige S, Nakanishi M, Natsuizaka M, Suda G, Sho T, Hatanaka K, Matsuno Y, Yokoo H, Kamiyama T, Taketomi A, Fujii G, Tashiro K, Hikiba Y, Fujimoto M, Asaka M, Maeda S. Heat shock factor 1 accelerates hepatocellular carcinoma development by activating nuclear factor κB/mitogen-activated protein kinase. Carcinogenesis. 2013. Feb;35(2):272-81. 2013.
  9. Nakagawa K, Uehata Y, Natsuizaka M, Kohara T, Darmanin S, Asaka M, Takeda H, Kobayashi M.
    The nuclear protein Artemis promotes AMPK activation by stabilizing the LKB1-AMPK complex.
    Biochem Biophys Res Commun. 2012 Nov 2;427(4):790-5.
  10. Naganuma S, Whelan KA, Natsuizaka M, Kagawa S, Kinugasa H, Chang S, Subramanian H, Rhoades B, Ohashi S, Itoh H, Herlyn M, Diehl JA, Gimotty PA, Klein-Szanto AJ, Nakagawa H.
    Genetic Notch inhibition reveals cyclin D1 and Wnt signaling in invasive esophageal squamous cell carcinoma.
    Am J Cancer Res. 2012. 2012;2(4):459-75.
  11. Natsuizaka M, Naganuma S, Kagawa S, Ohashi S, Ahmadi A, Subramanian H, Chang S, Nakagawa K, Ji X, Liebhaber SA, Klein-Szanto AJ, Nakagawa H.
    Hypoxia induces IGFBP3 expression in esophageal squamous cancer cells through polysome-enriched HIF-1α mediated mRNA transcription and continuous protein synthesis.
    FASEB J. 2012 Jun;26(6):2620-30.
  12. Kalabis J, Wong GS, Vega ME, Natsuizaka M, Robertson ES, Herlyn M, Nakagawa H, Rustgi AK.
    Isolation and characterization of mouse and human esophageal epithelial cells in 3D organotypic culture.
    Nat Protoc. 2012 Jan 12;7(2):235-46.
  13. Ohashi S*, Natsuizaka M*, Naganuma S*, Kagawa S, Kimura S, Itoh H, Kalman RA, Nakagawa M, Darling DS, Basu D, Gimotty PA, Klein-Szanto AJ, Diehl JA, Herlyn M, Nakagawa H. (* co-first authors);
    A Notch3-mediated squamous cell differentiation program limits expansion of EMT competent cells that express the ZEB transcription factors.
    Cancer Res. 2011 Nov 1;71(21):6836-47.
  14. Ohashi S. Natsuizaka M. Nakagawa H.
    MMP7 and activation of IGF1R: A new insight into anti-EGFR therapeutic resistance in metastatic colorectal cancer.
    Cancer Biol Ther. 2011 Jan 15;11(2):184-7.
  15. Hige S, Yamamoto Y, Yoshida S, Kobayashi T, Horimoto H, Yamamoto K, Sho T, Natsuizaka M, Nakanishi M, Chuma M, Asaka M.
    Sensitive assay for the quantification of hepatitis B virus mutants by a minor groove binder probe and peptide nucleic acids.
    J Clin Microbiol. 2010 Dec;48(12):4487-94.
  16. Ohashi S, Natsuizaka M, Yashiro-Ohtani Y, Kalman RA, Nakagawa M, Wu L, Klein-Szanto AJ, Herlyn M, Diehl JA, Katz JP, Pear WS, Seykora JT, Nakagawa H.
    NOTCH1 and NOTCH3 Coordinate Esophageal Squamous Differentiation Through a CSL-Dependent Transcriptional Network.
    Gastroenterology. 2010 Dec;139(6):2113-23.
  17. Natsuizaka M, Ohashi S, Kalman RA, Klein-Szanto AJ, Herlyn M, Diehl JA, El-Deiry WS, Nakagawa H.
    Insulin-like growth factor binding protein-3 promotes transforming growth factor-β1-mediated epithelial-to-mesenchymal transition and motility in transformed human esophageal cells.
    Carcinogenesis. 2010 Aug: 31(8):1344-53.
  18. Ohashi S, Natsuizaka M, Wong GS, Michaylira CZ, Grugan KD, Stairs DB, Kalabis J, Vega ME, Kalman RA, Nakagawa M, Klein-Szanto AJ, Herlyn M, Diehl JA, Rustgi AK, and Nakagawa H:
    EGFR and mutant p53 expand esophageal cellular subpopulation capable of epithelial-to-mesenchymal transition through ZEB transcription factors.
    Cancer Res. 2010 May 15;70(10):4174-84.
  19. Lee JJ*. Natsuizaka M*, Ohashi S, Wong G, Takaoka M, Michaylira CZ, Budo D, Tobias JW, Kanai M, Shirakawa Y, Naomoto Y, Klein-Szanto A, Haase VH and Nakagawa H (* co-first authors):
    Hypoxia activates the cyclooxygenase-2-Prostaglandin E synthase axis.
    Carcinogenesis. 2010 Mar;31(3):427-34.
  20. Natsuizaka M. Nakagawa H:
    Can Stressed Blood Cells Tell Cancer Risk in Inflammatory Bowel Diseases?
    Gastroenterology. 2009; 137(6); 2174-2175.
  21. Natsuizaka M, Kudo M, Suzuki M, Takano M, Tsuyuguchi M, Kawamura N, Noguchi S, Wada A, Nakata M, Ogasawara M, Kiyama Y, Asaka M, Kasai M:
    Diffuse large B-cell lymphoma with massive portal vein tumor thrombosis in a patient with alcoholic cirrhosis: a case report and literature review.
    Intern Med. 2009;48(10):805-8.
  22. Chuma M, Hige S, Nakanishi M, Ogawa K, Natsuizaka M, Yamamoto Y, Asaka M:
    8-Hydroxy-2‘-deoxy-guanosine is a risk factor for development of hepatocellular carcinoma in patients with chronic hepatitis C virus infection.
    J Gastroenterol Hepatol. 2008;23:1431-6.
  23. Natsuizaka M, Ozasa M, Darmanin S, Miyamoto M, Kondo S, Kamada S, Shindoh M, Higashino F, Suhara W, Koide H, Aita K, Nakagawa K, Kondo T, Asaka M, Okada F, Kobayashi M:
    Synergistic up-regulation of Hexokinase-2, glucose transporters and angiogenic factors in pancreatic cancer cells by glucose deprivation and hypoxia.
    Exp Cell Res. 2007;313:3337-48.
  24. Cui H, Darmanin S, Natsuisaka M, Kondo T, Asaka M, Shindoh M, Higashino F, Hamuro J, Okada F, Kobayashi M, Nakagawa K, Koide H, Kobayashi M:
    Enhanced expression of asparagine synthetase under glucose-deprived conditions protects pancreatic cancer cells from apoptosis induced by glucose deprivation and cisplatin.
    Cancer Res. 2007;67:3345-55.
  25. Miseki T, Kawakami H, Natsuizaka M, Darmanin S, Cui HY, Chen J, Fu Q, Okada F, Shindo M, Higashino F, Asaka M, Hamuro J, Kobayashi M:
    Suppression of tumor growth by intra-muscular transfer of naked DNA encoding adrenomedullin antagonist.
    Cancer Gene Ther. 2007;14:39-44.
  26. Natsuizaka M, Omura T, Akaike T, Kuwata Y, Yamazaki K, Sato T, Karino Y, Toyota J, Suga T, Asaka M:
    Clinical features of hepatocellular carcinoma with extrahepatic metastases.
    J Gastroenterol Hepatol. 2005;20:1781-7.
  27. Natsuizaka M, Hige S, Ono Y, Ogawa K, Nakanishi M, Chuma M, Yoshida S, Asaka M:
    Long-term follow-up of chronic hepatitis B after the emergence of mutations in the hepatitis B virus polymerase region.
    J Viral Hepat. 2005;12:154-9

国際学会(筆頭演者のみ)

  1. Natsuizaka M, Maehara O, Sato F, Kubota Y, Suda G, Itoh J, Tsunematsu S, Tsukuda Y, Terashita K, Nakai M, Sho T, Ogawa K, Ohnishi S, Sakamoto N.
    A pivotal role of KLF5 in regulation of cancer stem-like cells in hepatocellular carcinoma.
    AASLD the LIVER meeting 2014. Boston, Nov 7-11, 2014.(ポスター発表)
  2. Natsuizaka M, Kaimila B, Kubota Y, Hatanaka Y, Marukawa K, Terashita K, Sato F, Ohnishi S, Suda G, Ohashi S, Kagawa S, Whelan K, Rustgi AK, Nakagawa H, Sakamoto N.
    EGFR inhibitors eliminate esophageal cancer stem cells by suppressing epithelial-mesenchymal transition.
    Digestive Disease Week and the 115th annual meeting of the American Gastroenterological Association, Chicago, May 3-6, 2014.(ポスター発表)
  3. Natsuizaka M, Kagawa S, Whelan K, Ohashi S, Ohnishi S, Suda G, Sakamoto N, Rustgi AK, Nakagawa H.
    TGF-β in tumor microenvironment changes a switch of Notch1-mediated signaling to maintain cancer stem-like cells in esophageal squamous cell carcinoma.
    An AACR special conference on cellular heterogeneity in the tumor microenvironment. San Diego, Feb 26 - Mar 1. 2014.(ポスター発表)
  4. Natsuizaka M, Kagawa S, Whelan KA, Chang S, Kinugasa H, Ohashi S, Naganuma S, Sakamoto N, Klein-Szanto AJ, Gimotty PA, Diehl JA, Herlyn M, Rustgi AK, Nakagawa H.
    Transforming Growth Factor-beta and Notch1 Cooperate to Repress NOTCH3 to Facilitate Epithelial-Mesenchymal Transition and Tumor Initiating Capability in Esophageal Squamous Cell Carcinoma.
    Digestive Disease Week and the 114th annual meeting of the American Gastroenterological Association, Orlando, May 18-21, 2013.(口演発表)
  5. Natsuizaka M, Naganuma S, Kagawa S, Ohashi S, Whelan KA, Chang S, Rhim AD, Klein-Szanto AJ, Diehl JA, Herlyn M and Nakagawa H:
    A functional interplay between TGF-β and Notch signaling determines esophageal cancer cell fates.
    Digestive Disease Week and the 113th annual meeting of the American Gastroenterological Association, San Diego, May 19-22, 2012.(口演発表)
  6. Natsuizaka M, Chang S, Kagawa S, Naganuma S, Ohashi S, Whelan KA, Wong GS, Vega ME, Yang Y, Gimotty PA, Diehl JA, Herlyn M and Nakagawa H:
    CD44 defines highly plastic esophageal squamous cancer cells with tumor initiating capabilities.
    Digestive Disease Week and the 113th annual meeting of the American Gastroenterological Association, San Diego, May 19-22, 2012.(口演発表)
  7. Natsuizaka M, Nakagawa K, Naganuma S, Kinugasa H, Subramanian H, Ji X, Klein-Szanto AJ, Liebhaber SA, Diehl JA, Rustgi AK and Nakagawa H:
    Hypoxia induces IGFBP3 by HIF-1α-dependent transcription through a novel hypoxia-response element and privileged mRNA translation in the polysomes.
    Digestive Disease Week and the 113th annual meeting of the American Gastroenterological Association, San Diego, May 19-22, 2012.(口演発表)
  8. Natsuizaka M, Ohashi S, Naganuma S, Kalman RA, Ohyama A, Rhoades B, Vega ME, Stairs, DB, Klein-Szanto AJ, J. Diehl A, Basu D, Herlyn M, Pear WS and Nakagawa H:
    Notch regulates squamous differentiation, cell plasticity and tumor heterogeneity in esophageal carcinoma.
    The 102th Annual Meeting of American Association for Cancer Research, Orlando, FL, April 2-6, 2011.(ポスター発表)
  9. Natsuizaka M, OhashiS, Ahmadi A, Chang S, Nakagawa K, Wong GS, Miyamoto N, Grugan KD, Klein-Szanto AJ, Herlyn M, Diehl JA, Nakagawa H.
    IGFBP3 suppresses reactive oxygen species and cellular senescence response to facilitate TGF-β-mediated EMT.
    Digestive Disease Week and the 112th annual meeting of the American Gastroenterological Association, Chicago, IL, May 7-10, 2011.(口演発表)
  10. Natsuizaka M, Kalman RA, Ohashi S, Takaoka M, Klein-Szanto AJ, El-Deiry WS, Diehl JA, Herlyn M and Nakagawa H: IGF-independent regulation of EMT by insulin-like growth factor binding protein-3 in transformed human esophageal cells.
    101st AACR Annual Meeting 2010, Washington, DC, April 17-21, 2010.(ポスター発表)
  11. Natsuizaka M, Kobayashi M. Asaka M.
    Regulation of hypoxia-inducible factor-1α by prolyl hydroxylases and a factor inhibiting HIF.
    Keystone Symposium on “Hypoxia and Development, Physiolosy and Disease”, Breckenridge, CO, January 16-21, 2006.(ポスター発表)